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Colin McRae: DiRT 2 (2009) PC Hack Tool
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Sirtuin 3 (SIRT3) is a member of the sirtuin family of nicotinamide adenine dinucleotide (NAD)-dependent enzymes. Previously, we have reported that ectopic expression of SIRT3 suppresses the proliferation of endometriotic tissues and the development of endometriosis in mice. However, the molecular mechanism remains unclear. The aim of this study was to investigate the molecular mechanism underlying the protective role of SIRT3 in endometriosis development. Mice were divided into control, endometriosis (EM) and SIRT3-overexpressing EM (SIRT3-EM) groups. Rats were used to assess the effect of intratumoral injection of adenovirus containing SIRT3 (Ad-SIRT3) on the development of endometriosis in the EM group. After induced by EM or Ad-SIRT3, the expression and acetylation level of adenomatous polyposis coli (APC), an important tumor suppressor gene, in the endometriotic lesions were investigated, and the effect of SIRT3 on APC acetylation was explored. The expression levels of APC and SIRT3 were significantly reduced in EM and Ad-SIRT3 mice compared with controls. APC acetylation levels were remarkably increased in EM and Ad-SIRT3 mice compared with controls. Ad-SIRT3 markedly increased APC and SIRT3 expression, but decreased APC acetylation, in lesions of EM mice. In in vivo and in vitro studies, APC expression levels, but not the APC acetylation levels, were downregulated by SIRT3 overexpression. These data suggested that overexpression of SIRT3 suppresses the development of endometriosis in mice and attenuates APC deacetyl